ILL Deuteration Laboratory
Institut Laue-Langevin
The Deuteration Laboratory (D-Lab) is run as a user platform and is part of the ILL Life Sciences Group in Grenoble, France. It allows users in the area of life sciences and structural biology to seek tailor-made deuterated biomolecules in support of neutron scattering, protein crystallography, dynamics and reflectometry. The D-Lab is one of the many technical platforms of the Partnership for Structural Biology (PSB) located on the EPN campus in Grenoble.
Deuteration capabilities
- Recombinant protein expression in bacteria and yeast
- Deuteration of whole organisms (bacteria, yeast, algae)
- Peptide synthesis by solid phase
- Expertise in large crystal growth
- Labelled biomass, from which the target biomolecule can be extracted, is supplied to the user.
- Some purified model proteins can be requested : Maltose-Binding Protein, Human Serum Albumin, Myoglobin, Pfu Rubredoxin, Alpha-synuclein, Lysozyme.
- Small deuterated molecule of biological interest (cholesterol, campesterol) produced in vivo.
- The D-lab is providing the biomass to the ILL Lipid-Lab for the purification of lipids.
Specialities
- High-cell density culture
- Fermenter
- Various deuteration regimes (perdeuteration, matchout deuteration)
Key molecules
- Proteins
- Biomass
- Plasmid DNA
User access
User program
Yes
There is no call for D-Lab proposal applications and these can be submitted at any time during the year. Applicants should contact the D-Lab team before submitting proposals to discuss technical feasibility.
Access modes
Peer review
Access to the D-Lab is gained through a rapid peer-review proposal system and open to all the ILL member countries, regardless of where the neutron scattering study is carried out. It is also available to users from non-member countries - although a contribution to the costs may be requested.
Details
Address
Institut Laue-Langevin
CIBB building
Campus EPN
Grenoble
France
Contact
ILL D-Lab co-responsibles